Tapas Kumar Ghose
Lecturer, Botany
PhD: University of Nottingham, UK (1988, in Vitro Genetic Manipulation of Forage Legumes)

Research interest

DNA Markers for Quality Traits of Aromatic Rice Suitable for West Bengal for Rice:

We have identified around 100 rice lines, including Basmatis, which maintain aroma in West Bengal; DNA fingerprinting of 65 of these cultivars has been completed. Crossing and F2 segregation analysis show a single recessive gene controls the expression of aroma. A permanent mapping population of 700 recombinant inbred lines (RILs) has been developed from the non-aromatic/aromatic cross combination IR-72/Pusa Basmati-1. Using phenotyped F5 individuals of this cross, four SSLP (simple sequence length polymorphism) markers were identified to be linked with aroma on chromosome 8. The next target to map is cooked kernel elongation.

Rice Landraces of West Bengal:

We have collected around 250 rice landraces from different districts of West Bengal and have characterized the agro-morphology of 100 of the lines using 65 traits (Standard Evaluation System, IRRI). Our collection includes the "dual-seeded" landraces Jugal and Dui-Satin. Preliminary observations indicate that in Jugal, the gynoecium is reiterated 5 to 6 times before fertilization and consequent filling in of the endosperm.

Name of Group Members

• Narottam Dey
• Sudarsana Biswas
• Mitu De
• Tathagata Roychowdhury
• Santi Ranjan Dey

Important publications (fifteen)

1. Maji, A. K., Ghose, T. K. and Lohia, A. 1999. Use of AFLP DNA fingerprinting to differentiate pathogenic strains of Entamoeba histolitica (Schaudinin). J. Parasite Diseases. 23(2): 77-79.
2. Bhattacharya, S., Das, B., Ghose, T. K. and Bhattacharya, S. 1999. Investigation on seed germination of Nyctanthis arbortristis (Oleaceae) in relation to the total phenol content. Seed Sci Technol. 27(1): 321-327.
3. Lonsdale, D. M., Allen, R. L., Belostotsky, D., Ghose, T. K., Harvey, A. J., Rogers, H. J., Tebbut, S. J. and Trick, M. 1995. An analysis of the relative activities of a number of promoter constructs from genes which are expressed during the late pollen development as determined by particle bombardment. Plant Cell Reports. 15(1-2): 154-158.
4. Sen, J., Poddar, P., Murthy, J. S., Saha, P. K. and Ghose, T. K. 1994. Screening and breeding of aromatic rice suitable for South Bengal (West Bengal). RICE BIOTECH QUARTLY, 21: 15.
5. Baulcombe, D. C., Ghose, T. K., Cejedo, J. and Stabel, P. 1992. Gibberellin regulated genes in wheat. Annual Report 1991. John Innes Centre.
6. Cejedo, J., Ghose, T. K., Stabel, P. and Baulcombe, D. C. 1992. Analysis of the gibberellin responsive promoter of a cathepsin B-like gene from wheat. Plant Molecular Biology, 20: 849-856.
7. Ghose, T. K., Stabel, P., Cejedo, J. and Baulcombe, D. C. 1992. Functional analysis of a GA regulated wheat a-Amylase promoter in oat aleurone layers using microprojectile bombardment. Lecture presentation, The Biochemical Society Meeting, Lancaster, U. K.
8. Baulcombe, D., Cejudo, J., Ghose, T. K., Stabel, P. and Fincher, J. 1991. Analysis of Gibberellin Responsive Genes in Cereal Aluron Layer. Lecture presentation, The Biochemical Society Meeting, No. 640., Edinburgh, U. K.
9. Cejudo, J., Stabel, P., Ghose, T, K., and Baulcombe, D. C. 1991. Identification of wheat protinase promoter regulated by gibberellin in oat aluron layer. Third International Congress, International Society for Plant Molecular Biology, Tucson, U. S. A.
10. Golds, T. J., Lee, J. Y., Hussain, T., Ghose, T. K. and Davey, M. R. 1991. Agrobacterium rhizogenes mediated transformation of the forage legume Medicago sativa and Onobrychis viciifolia . J. Exp. Bot., 42: 1147-1157.
11. Slakeski, N., Xu, P., Wang, J., Ghose, T. K., Baulcombe, D. C. and Fincher, G. B. 1991. Molecular analysis and differential expression of the b- glucan endohydrolase gene family in barley. In, Munch, L. (ed.) Proceedings of the sixth International Barley Genetics Symposium, 1: 84.
12. Hammat, N., Lister, A., Blackhall, N. W., Gartland, J., Ghose, T. K., Gilmour, D. M., Power, J. B., Davey, M. R. and Cocking, E. C. 1990. Selection of plant heterokaryon from diverse origin by flow cytometry. Protoplasma, 190: 340-344.
13. Davey, M. R., Ghose, T. K., Golds, T. J., Lee, J. Y., and Gartland, K. M. A. 1987. Foreign genes and plant development. In, Boccon-Gibbol, J., Benbadis, A. and Short, K. C. (eds.) Cell Culture Techniques applied to Plant Production and Plant Breeding, Lecture Proceedings, I. A. P. T. C., Angers, 8-9 October, 1987.
14. Hammatt, N., Ghose, T. K., and Davey, M. R. 1986. Regeneration of legumes,. In, Vasil, I. K. (ed.) Cell Culture and Somatic Cell Genetics of Plants, Academic Press, 3: 67-95.
15. Davey, M. R., Jotham, J. P., Gilmour, D. M., Ghose, T. K. and Rybcjynskl, J. J. 1986. Development of protplast systems suitable for forage legume. In, Withers, L. W. and Alderson, P. G. (eds.) Plant Tissue Culture and Its Agricultural Applications. Butterworths, U. K. pp. 337-348.