|
Dibyendu N. Sengupta
Reader, Botany
PhD: University of Kalyani (1981) Present research activities
1.
The ddNTP- sensitive DNA Pol beta type enzyme from plant system has been
purified
and
functional analysis has been done on the enzyme from Rice and Vigna Mungo.
It’s role in endoduplication has been established.
2.
Salinity Stress in Rice: - ABA inducible transcription factor was found to be
very low in high yielding but salt sensitive rice cultivars but very high level
of ABA inducible factor was detected from the lamina and roots of salt tolerant
rice cultivars.. Antibody against OSBz8 was made and the protein OSBz8 was
detected poorly in salt sensitive and huge amount in salt tolerant (Nonabokra,
Pokkali) rice cultivars. Rab16a gene expression has been studied also. The gene
for Rab16a has been overexpressed in tobacco and the transgenics (at T1 ans T2
generation) were found to tolerate even 300mM NaCl. Similarly Samdecarboxylase
(SAMdC) cDNA was overexpressed in transgenic tobacco and the plants were found
to tolerate salinity stress.
3.
Fruit ripening of Tomato has been studied by analyzing the
expression of Le-MADS-RIN gene. The target genes for this transcription factor
has been identified and Light inducible cis-acting elements has been
identified from the 5’end of it’s gene. Work is going on to find out the gene
is light inducible. Fruit ripening in Banana has been studied in detailed by
analysis of the fruits from the variety Cavendish, Rasthahi, Kanthali, Poovan
and Monthan. The expression of the genes for ACC synthase, ACC oxidase and
sucrose-phosphate – synthase has been studied in detailed. In addition fruit
ripening in Tomato, Papaya, Water melon, etc, have been compared and the
production of lycopene or β-carotene have been studied by monitoring the
expression of three important genes like phytoene synthase, phytoene desaturase
and lycopene-β-cyclase.
Contact
dibyendu@bosemain.boseinst.ac.in
|
