Transcriptional and post transcriptional regulation of gene expression
during plant stress response
During biotic stress response in plants a series of signalling cascade
are activated. These pathways lead to the activation/repression of
transcription factors (TFs) that can regulate expression of defence
Reprogramming of gene expression cascade adds robustness and specificity
towards the stress response. microRNA (miRNA)-mediated
posttranscriptional regulation plays a crucial role in this process.
Expression of various miRNAs is altered immediately after perception of
stress signal by the cellular membrane.
These regulators (miRNAs) can lead to the differential regulation of
cognate transcripts that can boost the immune response. On the other
the pathogen can perturb this resistance-signalling mechanism and the
plant becomes susceptible.
However, very little is known about how the stress-signal from membrane
is transmitted for the regulation of the regulator which is the miRNA.
In addition some of the stress-responsive miRNAs may have role in other
physiological processes and expression level of these miRNAs is altered
under different conditions. This raises the question, how a miRNA
expression level and targeting will be affected when a plant is
undergoing physiological changes (e. g. developmental processes) and
then exposed to a stress? To answer these questions one has to
understand the molecular mechanisms of miR gene regulation and
homeostasis of these vital regulatory molecules in plant.
Using genomics, transcriptomics,
molecular biology and biochemical tools we are investigating on these
Analysis of small RNA transcriptome changes of tomato plant upon
perception of stress stimuli and signalling mechanism that leads to
the regulation of miR-genes.
Studies on the signalling crosslink involved in the regulation of
miR-genes under simultaneous physiological and stress cues.
Elucidation of the role of membrane anchored transcription factors
(MTFs) in transmission of stress signal from membrane to the
Ms. Sonali Roy,
Ms. Aindrila Chatterjee,
Ongoing Research Project:
Regulation of microRNA transcription and biogenesis by fungal-stress in
tomato, DBT, India.
Danesh SM*, Kundu P*,
Stefani E and Toro L, Distinct
transcriptional regulation of human large Conductance voltage- and
calcium-activated K+ channel Gene (hslo1) by activated
estrogen receptor alpha and c-src, J Biol Chem. 2011 Sep
9;286(36):31064-71. *, Equal Authorship.
Li M, Tanaka Y, Alioua A, Wu Y, Lu R,
Kundu P, Sanchez-Pastor
E, Marijic J, Stefani E, Toro L., Thromboxane A2 receptor and MaxiK-channel
intimate interaction supports channel trans-inhibition independent
of G-protein activation. Proc Natl Acad Sci U S A. 2010 Nov
Ciobotaru A, Foroughi S, Toro L, Stefani E, Eghbali M. Hormonal
regulation of cardiac KCNE2 gene expression. Mol Cell Endocrinol.
2008 Sep 24;292(1-2):50-62.
Lu R, Alioua A, Kumar Y,
Kundu P, Eghbali M, Weisstaub NV, Gingrich JA,Stefani E,
Toro L. c-Src tyrosine kinase, a critical component for 5-HT2A
receptor-mediated contraction in rat aorta. J Physiol. 2008 Aug
Alioua A, Lu R, Kumar Y, Eghbali M,
Kundu P, Toro L,
Stefani E. Slo1 caveolin-binding motif, a mechanism of
caveolin-1-Slo1 interaction regulating Slo1 surface expression. J
Biol Chem. 2008 Feb 22;283(8):4808-17.
Alioua A, Stefani E and Toro L, Regulation of mouse slo gene
expression: multiple promoters, transcription start sites, and
genomic action of estrogen., Journal of Biological Chemistry, 2007
Sep 14; 282(37):27478-92.
Raychaudhuri S, Tsai W, Dasgupta A., Shutoff of RNA polymerase II
transcription by poliovirus involves 3C protease-mediated cleavage
of the TATA-binding protein at an alternative site:incomplete
shutoff of transcription interferes with efficient viral
replication. Journal of Virology, 2005 Aug; 79(15):9702-13.
Jhaveri R, Kundu P,
Shapiro AM, Venkatesan A, Dasgupta A., Effect of heptitis C virus
core protein on cellular gene expression: specific inhibition of
cyclooxygenase 2., The Journal of
Infectious Diseases. 2005 May 1;191(9):1498-506. Epub 2005
Banerjee R, Weidman MK, Echeverri A,
Kundu P, Dasgupta
A. Regulation of
poliovirus 3C protease by the 2C polypeptide. 2004, Journal of
Weidman MK, Sharma R, Raychaudhuri S,
Kundu P, Tsai W,
Dasgupta A. The
interaction of cytoplasmic RNA viruses with the nucleus. 2003, Virus
Research. Sep;95(1-2):75-85. Review. Erratum in: Virus Res. 2004
Mandal S, Kundu P, Roy B
and Mandal R K,
Precursor of the inactive 2S seed storage protein from the Indian
mustard Brassica juncea is a novel trypsin inhibitor. 2002, Journal
of Biological Chemistry, 277 (40), 37161-37168.
A. Alioua., R. Lu, Y. Kumar, J. Ou., E. Stefani, and L. Toro. BK
Channels: Regulation of Expression and Physiological Impact, In:
Structure, Function and Pharmacology of Neuronal Voltage-Gated Ion
Channels. 2009, Eds. V. Gribkoff and G. Kaczmarek. Wiley Publishers.
Alioua, A., Kundu, P., Lu, R., Kumar, Y., Ou, J.,
Stefani, E., and Toro, L. Structure and Function of Ion Channels:
Large Conductance Calcium-activated Potassium Channels. In Squire,
L. R., editor. 2007, New
Encyclopedia of Neuroscience, Elsevier Ltd., Oxford, UK. In
Dasgupta A., Yalamanchili P., Clark M., Kliewer S., Fradkin L.,
Rubinstein S., Das S., Shen Y., Weidman K., Banerjee R., Datta U.,
Igo M., Kundu P., Barat
B. and Berk A. J.
Effects of Picornavirus proteinases on host cell transcription.
2002, Molecular Biology of Picornaviruses, pp 321-335, eds.
B.L.Semler and E. Wimmer, ASM Press, Washington DC
and Mandal R. K., Transgenic approaches for producing
plants. 2001, Proceedings of Indian National Science Academy
B 67, 53-80.
Division of Plant Biology
P 1/12 CIT Scheme VII (M)
Kolkata, West Bengal, 700 054
Telephone: (91)(33)2569 3298 or (91)(33)2355 9544 ext. 298