regulation” during plant response to environmental or development clues
Plants constantly modify their physiological
processes in response to developmental and environmental cues.
reprogramming of cell differentiation in response to stimuli requires
alteration of gene expressions, posttranscriptional and/or translational
modifications of factors. Regulation of gene
expression needs to occur in the context of chromatin. Change in
chromatin landscape is therefore the rate-limiting step for such
eukaryotes, accessibility of
DNA to transcription machinery is affected
by the degree of DNA packaging into chromatin structure. The central
question therefore is to understand how gene expression is precisely
regulated to give rise to the correct response during a process.
Epigenetic effects such as DNA methylation or histone modification have
been increasingly shown to play a critical role in global gene
regulation. The common outcome of epigenetic regulation is the
modification of the local chromatin structure which leads to the change
in accessibility of DNA sequence to the regulatory factors.
Plant cells after
perceiving the stress signals through various sensors, transduce the
signal by various pathways through many second messengers, plant
hormones, signal transducers and transcriptional regulators function.
This signal ultimately leads to regulation of many
stress-inducible gene products.
Some stress-inducible genes encode functional proteins
that are directly involved in stress tolerance
such as molecular chaperons, LEA proteins, metabolic enzymes etc.
Other stress-inducible genes encode regulatory proteins, such as signal
transducers, transcription factors etc form positive and negative
feedback loops to regulate stress responses.
As a whole it is clear that spatio-temporal expression of these
stress-inducible genes is the key for the immediate stress response and
ultimately tolerance to the plant.
Inductions of these stress-inducible genes are under the
tight transcription regulation which is guided by post-transcriptional
and post-translational modifications and epigenetic regulation.
- Study the
role of ARID/HMGs a non-histone protein, in modulating chromatin
accessibility in plants
Investigating the epigenetic changes when plants are subjected to
abiotic stress signal.
Anindita Seal & Maitrayee DasGupta (1999). Autophosphorylation Dependent
Activation of a Calcium Dependent Protein Kinase from
Arachis hypogea Plant.
Plant Physiology 120:
A., Hazra A., Nag, R., Chaudhuri, S.
and DasGupta, M (2001). Exogenous auxin depletion renders
Arachis hypogea suspension culture sensitive to water loss without
affecting cell growth.
Plant Cell Reports. 20(6):
567 – 573
Chatterjee, Yaya cui, Shubho
Chaudhuri and Arun Chatterjee (2002) Identification of regulators of
hrp/hop genes of Erwinia
carotovora ssp. carotovora and
characterization of HrpLEcc(sigma LEcc), an
alternative sigma factor.
Molecular Plant Pathology
Shubho Chaudhuri, Lihua
Yanping Chen, and B. W.
Calcium/calmodulin up-regulates a cytoplasmic receptor-like kinase in
J. Biol. Chem.,
Bhattacharyya R, Chaudhuri S,
Chakrabarti P, Dasgupta M. (2006) Domain
analysis of a groundnut calcium-dependent protein kinase: nuclear
localization sequence in the junction domain is coupled with nonconsensus
calcium binding domains.
J. Biol. Chem.,
Shubho Chaudhuri, Tianbao Yang ,
Alfonso Munoz-Gutierrez , B. W.
Poovaiah, GIles Oldroyd. (2006) Legume nodulation independent of rhizobia is
induced by a modified calcium/calmodulin activated kinase lacking
Nature 441: 1149–1152
Shubho Chaudhuri, John J. Wyrick, and Michael
J. Smerdon. (2009)
Histone H3 Lys79 Methylation
is Required for Efficient Nucleotide Excision Repair in a Silenced Locus of
Nucleic acids research
Shubho Chaudhuri, Lihua Yang,
Liqun Du and B.W. Poovaiah (2010). Calcium/calmodulin-regulated
Receptor-like Kinase is a positive regulator of Cold tolerance in Plants.
J. Biol. Chem
- Maitrayee DasGupta and
Subho Chaudhuri (2001). CDPKS in
plant signaling networks- Progress in research on Groundnut CDPK.
“Signal transduction in plants”
(S.K. Sopory; R. Oelmuller and S. C. Maheshwari, Eds.). Kluwer academic
Press, Page 145-156.
Ongoing Research Project:
Investigating the role of ARID/HMG -
a novel high mobility group protein in modulation of chromatin structure in
plants. CSIR Project (PI: Dr. Shubho Chaudhuri)
Generation of salt-tolerant rice
plants: A translational approach. DBT project. (Co-ordinator: Prof.
A. N.Lahiri Majumder, Co-Investigator: Dr. Shubho Chaudhuri)