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Themes of research:
Ex situ conservation of genetic diversity and
looking for useful trait(s) from the wild gene pool
Wild gene pool is the
reserve currency for entire mankind and all the living organisms in general.
It is, therefore, our bounden duty to conserve, characterize, classify and
catalogue the wild germplasms.The gene pool of a species or
a population is the complete set of unique alleles that would be found by
inspecting the genetic material of every living member of that species or
population. A large gene pool indicates extensive genetic diversity, which
is associated with robust populations that can survive bouts of intense
selection. Genetic diversity is a characteristic of gene pools that
describes an attribute which is commonly held to be advantageous for
survival -- that there are many different versions of otherwise similar
organisms. While plant breeding techniques have been successful in
developing high-yielding varieties in certain crops, the process itself
threatens the genetic base. New plant varieties are usually derived from
crosses between close relatives, and these result in a "genetic bottleneck"
that can result in losing plant production and making plants more vulnerable
to pests and disease. Hunt for useful trait(s) from the wild relatives thus
is a continuous process for the plant scientists for further improvement of
the cultivated genotypes.
The focus of my research,
hence, is looking for useful trait(s) from primary, secondary as well as
tertiary gene pools by conventional, biotechnological and molecular
biological approaches for improvement of certain hitherto unexploited or
underexploited crop plant. Furthermore, the reservoir of these wild gene
pools, i.e., the threatened or endangered plants will be preserved by in
vitro approaches for the purpose of ex situ conservation.
Publications (Important ten)
1.
Gangopadhyay, G., Roy, S.K., Basu Gangopadhyay, S. and
Mukherjee, K.K. 2009. Agrobacterium-mediated genetic
transformation of pineapple var. Queen using a novel encapsulation-based
antibiotic selection technique. Plant Cell tissue and Organ Culture
97: 295-302 (Online version DOI 10.1007/s11240-009-9528-8).
2.
Roy, S.K., Gangopadhyay, G., Ghose, K., Dey, S., Basu, D. and
Mukherjee, K.K. 2008. A cDNA-AFLP approach to look for differentially
expressed gene fragments in dioecious pointed gourd (Trichosanthes dioica
Roxb.) for understanding sex expression. Current Science 94 (3): 381
- 385.
3.
Gangopadhyay, G., Roy, S. K., Ghose, K., Poddar, R.,
Bandyopadhyay, T., Basu, D. and Mukherjee, K.K. 2007. Sex detection
of Carica papaya and Cycas circinalis in pre flowering stage
by ISSR and RAPD. Current Science 92(4): 524-526.
4.
Gangopadhyay, G., Bandyopadhyay, T., Poddar, R., Basu
Gangopadhyay, S. and Mukherjee, K.K. 2005. Encapsulation of pineapple
micro shoots in alginate beads for temporary storage. Current Science
88(6): 972-977.
5.
Gangopadhyay, G., Bandyopadhyay, T., Modak, B.K., Wongpornchai,
S. and Mukherjee, K.K. 2004. Micropropagation of Indian pandan (Pandanus
amaryllifolius Roxb.), a rich source of 2-acetyl-1-pyrroline. Current
Science 87(11): 1589-1592.
6.
Gangopadhyay, G., Bandyopadhyay, T., Basu Gangopadhyay, S. and
Mukherjee, K.K. 2004. Luffa sponge – a unique matrix for
tissue culture of Philodendron. Current Science 86(2):
315-319.
7.
Gangopadhyay, G., Das, S. and Mukherjee, K.K. 2002.
Speciation in Chenopodium in West Bengal, India. Genetic Resources
and Crop Evolution 49: 503-510.
8.
Gangopadhyay, G., Das, S., Mitra, S.K., Poddar, R., Modak, B.K.
and Mukherjee, K.K. 2002. Enhanced rate of multiplication and rooting
through the use of coir in aseptic liquid culture media. Plant Cell
Tissue & Organ Culture 68(3): 301-310.
9.
Gangopadhyay, G., Basu, S. and Gupta, S. 1997. In
vitro selection and physiological characterization of NaCl- and mannitol
– adapted callus lines in Brassica juncea. Plant Cell Tissue &
Organ Culture 50: 161-169.
10.
Gangopadhyay, G., Basu, S., Mukherjee, B.B. and Gupta, S. 1997.
Effects of salt and osmotic shocks on unadapted and adapted callus lines of
tobacco. Plant Cell Tissue & Organ Culture 49: 45-52.
Full publication list
Submissions to GenBank, NCBI database
1.
Roy, S.K., Gangopadhyay, G.
and Mukherjee, K.K. 2009.
Zamia fischeri
clone ZFM1 male-specific RAPD marker genomic
sequence. GenBank Accession
GQ141708.
2.
Roy, S.K., Gangopadhyay, G.
and Mukherjee, K.K. 2009.
Zamia fischeri
clone ZFF1 female-specific RAPD marker genomic
sequence. GenBank Accession
GQ141709.
3.
Roy, S.K., Gangopadhyay, G.,
Ghose, K., Dey, S., Basu, D. and Mukherjee, K.K. 2006.
Understanding sex expression in Trichosanthes dioica Roxb.
GenBank Accessions EF192055 - EF192076, EF198868 - EF198876.
4.
Gangopadhyay, G., Roy, S.K.,
Ghose, K., Poddar, R., Bandyopadhyay, T., Basu, D. and Mukherjee, K.K.
2006. Cycas circinalis clone
CCM1 male-specific RAPD marker genomic sequence. NCBI Accession
DQ386640.
Patent
Filed a
patent (Application No. 770/Cal/99 dtd.
09.09.99) entitled “Downstream processing in micropropagation”.
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