M.Sc., M.S., Ph.D.
Division of Molecular Medicine
Scientist-In-Charge, Animal Research Facilty
Bose Institute, P 1/12, CIT Scheme VIIM, Kolkata-700 054,
Molecular Mechanisms of Germ-line Stem Cell Regulation:
Germ-line stem cells (GSCs), which can self-renew and generate
differentiated progeny, are unique stem cells in that they are solely
transmit genetic information from generation to generation. Through
the use of genetic techniques in Drosophila, Caenorhabditis elegans,
and mouse, exciting progress has been made in understanding
molecular mechanisms underlying interactions between stem cells and
niches. The knowledge gained from studying GSCs has provided an
intellectual framework for efining niches and molecular
dregulatory mechanisms for other adult stem cells. Our focus is to
study the conserved mechanisms underlying GSC self-renewal and
Molecular Signalling of Germ cell and Leydig cell Apoptosis by
Benzo(a)pyrene, an Environmental Toxicant:
Possible Protection by
Natural Aryl Hydrocarbon Receptor Antagonists:
The male reproductive abnormalities of benzo(a)pyrene have been
demonstrated in male
are mediated by the aryl hydrocarbon
receptor (AhR). This study
related to diminution of testicular steroidogenesis and
steroidogenic acute regulatory gene (StAR) expressions which is
responsible for mitochondrial cholesterol transport associated with
caspases expressions, glutathione depletion and germ cell/ Leydig
cell DNA damage coupled with p38MAP
following benzo(a)pyrene treatment.
The resveratrol (3,5,4'-trihydroxystilbene),
a plant antifungal agent or phytoalexin,
present in red wines, is a competitive inhibitor of AhR
ligands. Resveratrol binds AhR, and
inhibits the induction of DRE-driven transcription
by AhR ligands in
both ex vivo
and in vivo. Therefore,
If natural AhR antagonist could able to prevent such testicular
damage, so what is the molecular mechanism to prevent such damage?
Ageing Associated Testicular Gametogenic and Steroidogenic
In aged testis, ROS produced by endogenous cellular processes
resulting in attenuation of LH receptor signalling to adenylyl
cyclase may have a short-term effects on the intracellular transfer
of cholesterol to the mitochondria and
effects on the metabolism of cholesterol to testosterone. Therefore,
the focus of research is to study the steroidogenic processes in aged Leydig
cells in relation to the molecular mechanism of germ cell and Leydig
Molecular Signalling of Protein kinase-D and β-Catenin Interaction
in Cancer progression:
Protein kinase D (PKD), the founding member of a new family of serine/threonine
protein kinases occupies a unique position in the signal
transduction pathways initiated by DAG and PKC in normal and cancer cells. Whereas, β
-Catenin has been shown to function as a signalling molecule involved in
the process of development, proliferation and differentiation. Multiple
lines of evidences suggest that stabilization and subsequent nuclear
accumulation of β-Catenin are positively linked to various human
malignancies. Several recent studies
have also demonstrated β-Catenin as
a putative regulator of COX-2, suggesting β -Catenin as a potential
target for chemoprevention by anti-inflammatory substances. Moreover,
protein Kinase-D (PKD) and β -Catenin are emerging as the valuable
target for development of novel therapeutic approaches in
prostate/cervical/breast cancers in human. Therefore, presently we are
working on signal transduction pathways related to these valuable
Chemical Contraception of Pet Animals:
The study examined the efficacy and stress of single bilateral
intratesticular injection of a new drug preparation ChemisterisolTM
in different male domestic
animals for the induction of sterilization through apoptosis and
necrosis of testicular parenchyma and to explore the molecular
mechanism of action of this drug for the induction of chemical
sterilization in male.
Link Between Polycystic Ovary Syndrome and Both Type 1 and Type 2
Polycystic ovary syndrome (PCOS) and Type 2 diabetes mellitus (T2D)
are both obesity-related conditions that share epidemiological and
patho-physiological factors. Insulin resistance is a key factor
whereby obesity influences the expression of each condition.
However, the mechanisms by which insulin resistance contributes
towards the manifestation of PCOS and T2D differ in important ways:
compensatory hyperinsulinemia results in pleiotropic effects
including co-gonadotrophic stimulation of ovarian and adrenal
steroidogenesis; in T2D, insulin resistance contributes towards
β-cell exhaustion and ultimately to hypo-secretion of insulin with
resultant dysglycemia. The link between PCOS and Type 1 diabetes
mellitus is believed to implicate supra-physiological concentrations
of insulin within the systemic circulation. Further progression of
the obesity epidemic will ensure even greater prominence of
important obesity-related conditions such as PCOS and T2D. Research
continuing to gain a clearer understanding of the mechanisms linking
each condition should be a top priority.
1. Prof. Sanghamitra
Raha, Saha Institute of Nuclear Physics, Kolkata.
2. Prof. Parimal C.
Sen, Bose Institute, Kolkata
3. Prof. Sujoy Guha,
Indian Institute of Technology, Kharagpur
4. Prof. Narayan Jana,
Institute of Post-Graduate Medical Education & Research, Kolkata.
5. Prof. Baidyanath
Chakraborty, Institute of Reproductive Medicine, Kolkata.
6. Dr. Debasish
Bandopadhyay, University of Calcutta, Kolkata.
7. Dr. Anup Kr. Misra,
Bose Institute, Kolkata.
The Biography included in Americas “Who’s Who in Medicine &
DST-Fast-Track Young Scientist Scheme Award, 2010.
Young Scientist Travel Award, 2010 from ACC&D (Portland, Oregon,
Young Investigator Award, 2011 from Parsemus Foundation (San
Francisco, California, USA).
Nominated as “International Scientist of the Year, 2011” by
International Biographical Centre, Cambridge, U.K.
Scientific Award of Excellence for 2011" by American Biographical Institute,
San Francisco, California, U.S.A.
Translational Medicine: Current Research
International Journal of Plant Physiology & Biochemistry
Journal of Diabetes & Endocrinology
Journal of Toxicology and Environmental Health Sciences
1. Journal of
Endocrinology 2. Molecular Cellular Endocrinology 3.Toxicological Sciences
4.. International Journal of Andrology 5. Asian Journal of Andrology 6. Food
& Chemical Toxicology 7. Ecotoxicology & Environmental Safety 8. Fertility &
Sterility 9. Contraception 10. Reproductive Toxicology 11. Molecular
Cellular Biochemistry 12. Molecular Reproduction & Development 13. Journal
of Trace Elements in Medicine & Biology 14. Molecular Human Reproduction 15.
Life Sciences etc.
Member of Scientific Bodies:
1. The Science
Advisory Board, U.S.A.
2. Society for
the Study of Reproduction and Fertility
3. Society for Reproductive Biology and Comparative Endocrinology.
4. The Physiological Society
List of Important research publications:
P. Nandi, S.
P. C. Sen. Role of a 14 kD sperm protein p14 in maturation, acrosome
reaction and capacitation of caprine spermatozoa.
P.K. Samanta. Clinical evaluation of non-surgical Sterilization of male Cats
with Single intra-testicular Injection of Calcium Chloride.
BMC Veterinary Research
7 (2011) 39.
N. Jana, D.K. De & S. K. Guha. Ethanol induces mouse spermatogenic cell
apoptosis in vivo through over expression of Fas/Fas-L, p53 and caspase-3
along with cytochrome c translocation and glutathione depletion.
Molecular Reproduction & Development
77 (2010) 820-833.
P.K.Samanta & D.K. De. Nicotine diminishes testicular gametogenesis,
steroidogenesis and steroidogenic acute regulatory protein expression in
adult albino rats: possible influence on pituitary gonadotropins and alters
testicular antioxidant status.
116 (2010) 647-659.
I. Manna, & P.K. Samanta.
Protective effect of sodium selenite and zinc
sulfate on intensive swimming exercise- induced testicular gamatogenic and
steroidogenic disorders in mature male rats.
Applied Physiology, Nutrition,
Metabolism 33 (2008) 903-914.
P. Yin, X. Schiffer, P. Grammas, D.M. Stocco & X.J. Wang. Effect of
Chrysin on steroidogenesis and steroidogenic acute regulatory gene
expression in mouse MA-10 tumor Leydig cells.
Journal of Endocrinology 197 (2008) 315-323.
7. K. Jana
& P.K. Samanta. Strilization of male stray dog by single intratesticular
injection of calcium chloride.
Contraception 75 (2007) 390-400.
P.K. Samanta, I. Manna &
Effect of L - ascorbic acid supplementation on testicular oxidative stress
and reproductive dysfunctions in rats exposed to intensive exercise stress.
Reproductive Medicine and Biology 5
9. K. Jana,
S. Samanta, P.K. Samanta. The possible
estrogenic mode of action of sodium arsenite on pituitary- testicular
activities in adult albino rats.
Reproductive Biology and Endocrinology 4 (2006), 9- 22.
10. K. Jana
& P.K. Samanta. Evaluation of single
intra-testicular injection of calcium chloride for non-surguical
sterilization in adult albino rats.
Contraception 73 (2006), 289-300.
11. K. Jana,
D. Ghosh & P.K. Samanta. Evaluation of
single Intra-testicular injection of calcium chloride for non-surgical
sterilization of male Black Bengal goats. (Capra-hircus): a dose dependent
study. Animal Reproduction Science
86 (2005) 89-108.
I. Manna, K. Jana, P.K. Samanta,
Effect of different intensities of Swimming Exercise on testicular
oxidative stress and reproductive dysfunctions in mature male albino Wister
rats. Indian Journal of Experimental Biology,
42 (2004) 816-822.
I. Manna, K. Jana & P. K. Samanta.
Intensive swimming exercise induced oxidative stress and reproductive
dysfunctions in male wistar rats: Protective role of α- tocoferol
succinate. Canadian Journal of Applied
Physiology, 29 (2004) 172- 185.
I. Manna, K. Jana & P. K. Samanta.
Effect of intensive exercise-induced testicular gametogenic and
steroidogenic disorders in matured male wistar strain rats: A correlative
approach to oxidative stress. Acta
Physiologica, 178 (2003) 33-40.
P. K. Samanta & D. Ghosh. Dose dependent
response to an intratesticular injection of calcium chloride for induction
of chemosterilization in adult albino rats.
Veterinary Research Communications,
26 (2002) 651-663.
& P. C. Sen (2011)
Environmental Toxicants Induced Male Reproductive Disorders: Identification
and Mechanism of Action.
Toxicity & Drug Testing: